Red top or SST tube
0.5 mL serum
0.4 mL serum
Collect specimen in a red top or SST tube. Centrifuge, aliquot serum into plastic vial and refrigerate. Morning (8 a.m.) specimens are recommended. Indicate time of collection and whether specimen was collected pre- or post- metyrapone.
Collect specimen in a red top or SST tube. Morning (8 a.m.) specimens are recommended. Indicate time of collection and whether specimen was collected pre- or post- metyrapone.
Tuesday
3 - 9 days
Liquid Chromatography-Tandem Mass Spectometry (LC-MS/MS)
< or =18 years: <344 ng/dL, >18 years: 10-79 ng/dL.
Diagnostic workup of patients with congenital adrenal hyperplasia; Part of metyrapone testing in the workup of suspected secondary or tertiary adrenal insufficiency; Part of metyrapone testing in the differential diagnostic workup of Cushing syndrome.
There have been occasional reports of Addisonian crisis during 2-day metyrapone testing. For this reason, 2-day metyrapone testing probably should not be performed when plasma cortisol values are less than 3 mcg/dL.
Ethanol, estrogens (exogenous and pregnancy-related), barbiturates, valproic acid, phenytoin, and exogenous gluco-corticoids may cause impaired response to metyrapone.
Test sent to Mayo Medical Laboratories.
82634
20420