Lavender top tube
3 mL EDTA whole blood
1.5 mL EDTA whole blood
Collect specimen in a lavender top (EDTA) tube. Invert specimen after collection to prevent clotting. Send intact specimen; do not centrifuge. Refrigerate; do not freeze. Must include most recent CBC results (performed within 21 days of HGBE specimen collection).
Collect specimen in lavender top tube. Invert specimen after collection to prevent clotting. CBC testing must be performed in conjunction with HGBE test to aid in interpretation of results.
Monday - Friday
24 hours
High Pressure Liquid Chromatography (HPLC)
HGBN-A1: 96.0 - 98.0% of total hemoglobin; HGBN-A2: 2.3 - 3.4% of total hemoglobin; HGBN-F, 0-1 month: 46.0 - 81.0% of total hemoglobin; HGBN-F, 1-4 months: 9.4 - 61.0% of total hemoglobin; HGBN-F, 4-12 months: 1.3 - 22.0% of total hemoglobin; HGBN-F, 1 - 150 years: less than or equal to 2% of total hemoglobin. There are no established reference ranges for HGBN-C and HGBN-S. Pathologist interpretation of abnormal results provided.
Evaluate distribution of hemoglobins.
Numerous transfusions prior to HGBE testing may affect results. HGBE testing on newborns may be affected by high levels of fetal hemoglobin.
Testing includes Hemoglobins A1, A2, C, F and S. By ordering this test, the clinician acknowledges that a pathologist interpretation will be performed and billed as a separate charge if indicated. Confirmation of all new cases of hemoglobin variants and homozygous sickle samples will be performed in-house by capillary electrophoresis or by an outside reference laboratory at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. All confirmation testing will be performed on test systems different from initial test system, as per CAP requirements. Various sendout confirmation test sites may be utilized depending on the complexity or uniqueness of the hemoglobinopathy. Confirmation tests may include pathologist interpretation of results at an additional charge.
49322-1 (Hgb Fractionation); 4547-6, 42245-1, 42246-9, 44923-1, 44920-7; 78748-1 (Confirm)
83021
37917
83020
KA014
Green top (sodium or lithium heparin) tube is not acceptable. [4/08]