Clinical Lab Handbook

• Green top sodium heparin Vacutainer® tube
• (FULL)Yellow top ACD A or B Vacutainer® tube

• Whole blood: (FULL) Yellow top ACD A or B Vacutainer® tube
• Whole blood: 7-10 ml Green top sodium heparin tube

Whole blood: 3 ml added to Green top sodium heparin tube

The following information is required on a Hematopathology Consult requisition:

1. Relevant clinical history
2. Clinical or morphological findings and suspicions
3. Date and time of collection
4. Phone number and after hours pager number for ordering physician

• Collect blood in a green 7-10 ml sodium heparin Vacutainer® tube or yellow top ACD A or B Vacutainer® tube and transport at room temperature within 12 hours of collection.
• Include a copy of the patient's concurrently drawn White Blood Cell and Platelet counts, peripheral blood differential and an unstained peripheral blood smear, OR, concurrently collect a lavender EDTA Vacutainer® tube for a CBCD to be performed upon receipt of the specimen.

The following information is required on a Flow Cytometry requisition:

1. Relevant clinical history
2. Clinical or morphological findings and suspicions
3. Date and time of collection
4. Phone number and after hours pager number for ordering physician

• Collect blood in a yellow ACD A or B Vacutainer® tube or green 7-10 ml sodium heparin Vacutainer® tube and transport at room temperature within 4 hours of collection.
• A concurrent CBCD should also be ordered for the patient.

Monday - Friday, 8:00am - 4:30pm; Saturday 8:00am-4:30pm

8 - 48 hrs

Direct immunofluorescence using flow cytometric techniques.

LEUKB AND IMM reference ranges.docx *Pediatric Reference Ranges from Journal of Allergy and Clinical Immunology, vol. 112, No. 5, 11/2003 and studies performed at Michigan Medicine Flow Cytometry Laboratory. Absolute ranges are calculated utilizing absolute lymphocyte count ranges for the same age groups provided by the hematology laboratory.

Confirmation of diagnosis and immunophenotype in acute and chronic leukemias and lymphomas. See http://www.pathology.med.umich.edu/handbook/Tables/Flow_Cytometry_Panels.pdf for all Flow Cytometry Panels.

Requires an adequate number of viable cells for analysis. Improper collection or transport may result in decreased cell viability and cancellation of testing. Specimen transport should be arranged so that specimen will be received Monday-Friday and prior to noon on Saturday.

Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation. Additonal markers will be run if clinically indicated. Measurement of TdT may be an adjunct to the diagnosis of some leukemias and lymphoblastic lymphomas and will be run at the discretion of the hematopathologist at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation, contact the MLabs Client Services Center or Flow Cytometry Laboratory at 76-39420.

Z501-7

ACUTE: 88184, 88185 x23, 88189; NONPB: 88184, 88185 x14, 88188; COMBO: 88184, 88185 x22, 88189

ACUTE: 23033, 23041 x23; NONPB: 23033, 23041 x14; COMBO: 23033, 23041 x22

ACUTE: 88189.1, NONACUTE: 88188.3, COMBO: 88189.2

Lavender EDTA Vacutainer® tubes are acceptable but not preferred.

Green top with lithium heparin is unacceptable. Clotted peripheral blood specimens are unacceptable