Lavender top tube
5 mL EDTA (lavender) whole blood; 3 mL EDTA (lavender) bone marrow; Fresh/frozen cells in RPMI. Extracted DNA is also acceptable if extracted in a CLIA certified laboratory.
Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice.
Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice.
Monday - Thursday
2 - 7 days
Multiplex Polymerase Chain Reaction (PCR) with Capillary Electrophoresis
Interpretive report provided.
Mutations within exon 12 of the JAK2 gene occur in most cases of JAK2 V617F-mutation negative polycythemia vera. Testing for JAK2 exon 12 mutations may aid in the diagnosis of polycythemia vera, and is recommended in patients with JAK2 V617F-negative erythrocytosis. This test will qualitatively detect JAK2 exon 12 mutations in peripheral blood or bone marrow specimens with a sensitivity down to 2% mutant allele. This is a second order test and should be used only following a JAK2 V617F-negative result.
This test is not indicated for inpatient evaluation of acute thrombosis.
The limit of detection of this assay is 4% mutation-bearing cells. Mutations may not be detected in samples with a neoplastic burden below this level. In addition, this test will only detect insertions, deletions or K539L. Rare substitution mutations other than K539L (accounting for less than 5% of described JAK2 exon 12 mutations) will not be detected.
By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.
81279
NA114
G0452-26
The preferred specimen is whole blood or bone marrow collected in EDTA (lavender top). However, ACD (yellow top) and heparin (green top) may also be accepted. Previously extracted DNA may also be accepted.