1 Viral Transport Media (VTM) Acceptable Viral transport media: M4RT, UTM or BD-UVT
1 Viral Transport Media (VTM)
1 Viral Transport Media (VTM)
For blister, lesion, or vesicle, unroof and scrape the base of the lesion with a Dacron or rayon swab to collect fluid and cells. Place the swab into 1 Viral Transport Media (VTM). Ideally specimens should be collected within 3-4 days of the onset of symptoms but no greater than 7 days. Specimens collected from lesions in the acute or vesicular stage will yield a higher number of viruses. Avoid contamination with creams, ointments, lotions, alcohol, Betadine solution or blood due to the potential for PCR inhibition. Transport to the laboratory as soon as possible. Calcium alginate swabs are not acceptable.
Daily
6 hours
Polymerase Chain Reaction (PCR)
Not detected.
Rapid detection of Varicella zoster virus DNA in clinical specimens.
A negative test result does not rule out the presence of Varicella zoster virus. Results should be used in conjunction with other laboratory test results and the patient's clinical profile.
The Varicella zoster virus PCR is a real-time polymerase chain reaction (PCR) amplification and detection system that utilizes a bi-functional fluorescent probe-primer for the detection of Varicella zoster virus in lesion/vesicle swabs. The assay has been validated as a direct protocol using unprocessed specimens. A DNA internal control is used to monitor the process for reagent integrity and to detect PCR inhibition. Results should be used in conjunction with other laboratory test results and the patient's clinical profile
11483-5
87798
LA005
Specimens not received in Viral transport media (VTM).
Room temperature (15-25°C) ≤4 days Refrigerate (2-8°C) ≤14 days Freeze (-15 to -25°C) ≤90 days
7 days