Lavender top tube
5 mL EDTA (lavender) whole blood; 3 mL EDTA (lavender) bone marrow; Fresh/frozen tissue and fresh aspirates or body fluids; Fresh/frozen cells in RPMI; Fixed cytogenetic cell suspensions or pellets in Carnoy's fixative. Extracted DNA will be accepted from specimens that were not extracted from formalin-fixed specimens, if extracted in a CLIA certified laboratory. No minimum volume requirement for fluids.
Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh tissue (preferably 0.5cm3, sent in RPMI) and fresh aspirates or body fluids are acceptable. Refrigerate and send, preferably within 24 hours. Frozen tissue specimens – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fixed cytogenetic cell suspensions or pellets in Carnoy’s fixative. Refrigerate and send.
Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh tissue (preferably 0.5cm3, sent in RPMI) and fresh aspirates or body fluids are acceptable. Refrigerate and send, preferably within 24 hours. Frozen tissue specimens – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fixed cytogenetic cell suspensions or pellets in Carnoy’s fixative. Refrigerate and send.
Collect blood or bone marrow specimen in a lavender top (EDTA) tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection.
Monday - Friday
2 - 7 days
Multiplex Polymerase Chain Reaction (PCR) with Capillary Electrophoresis
Interpretive report provided.
The FLT3 gene encodes a cell surface receptor tyrosine kinase that is expressed on early hematopoietic stem cells. Activating mutations in FLT3 occur in approximately 25-30% of patients with acute myelogenous leukemia (AML). The mutation generally occurs as either an internal tandem duplication (ITD) within the juxtamembrane domain, or as a missense point mutation within the tyrosine kinase domain (TKD) at codon 835. This test detects both the FLT3-ITD and FLT3-TKD (codon 835) mutations. The presence of a FLT3-ITD mutation has been associated with a significantly poorer outcome in normal karyotype AML.
The limit of detection of this assay is 10% mutation-bearing cells. Mutations may not be detected in samples with a neoplastic burden below this level.
By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.
Z500-9
81245, 81246
NA010, NA011
G0452-26
The preferred specimen is whole blood or bone marrow collected in EDTA (lavender top). However, ACD (yellow top) and heparin (green top) may also be accepted.
Formalin-fixed paraffin-embedded specimens are not acceptable.